This is a link to the motif in the output of the particular motif discovery (e.g., MEME) or motif enrichment (e.g., CentriMo) program that reported it.

This is the significance of the motif according to the particular motif discovery (e.g., MEME) or motif enrichment (e.g., CentriMo) program that reported it.

Follow the link under the "Discovery/Enrichment Program" column for more information on how the significance value was derived.

Motifs reported by a motif discovery program (e.g., MEME) are compared with known motifs in a motif database specified by the user. This column lists the (up to) three most similar motifs. Only known motifs with TOMTOM similarity E-values of less than 1.0 to the discovered motif will be shown here. Clicking any of these links will show the TOMTOM results where all alignments can be viewed.

Motifs reported by a motif enrichment program (e.g., CentriMo) list the motif's name and a link to the motif's entry on the database website if it is available.

This graph shows the distribution of the best matches to the motif in the sequences as found by a CentriMo analysis.

The vertical line in the center of the graph corresponds to the center of the sequences.

Clicking on a motif's graph will take you to the CentriMo output with that motif selected for graphing.

Clicking here will show you all the motifs found by motif discovery or motif enrichment analysis that are significantly similar to the reported motif.

The additional motifs are shown aligned with the reported motif, sorted in order of significance of the motif according to the particular motif discovery (e.g., MEME) or motif enrichment (e.g., CentriMo) program that reported it.

To cluster the motifs MEME-ChIP does the following:

1. Start with no groups and all significant reported motifs.
2. Run TOMTOM with all significant reported motifs to determine pairwise similarity.
3. Group Highly Similar Motifs---
   While ungrouped motifs:

   Select most significant ungrouped motif.

   This is called the "seed" motif for the group and we will call the E-value of its seed motif the group's "significance".

   Form a new group from the seed motif and all other motifs that are not yet in a group and who are strongly similar to the seed motif (default: TOMTOM E-value ≤ 0.05).
4. Merge Groups---
   For each group (most significant to least significant), merge it with any less significant group if all of its motifs are weakly similar to the first group's seed motif (default: TOMTOM E-value ≤ 0.1).

Clicking here takes you to the CentriMo motif enrichment analysis with the results for this all the motifs in this group.

This lists links to related content, which may include:

• Motif Spacing Analysis--SpaMo results using this discovered motif as the "primary" motif, and each of the discovered motifs and motifs in any motif databases specified to MEME-ChIP as potential "secondary" motifs. SpaMo reports the secondary motifs whose occurrences are enriched at particular distances relative to the primary motif's occurrences in the input sequences.
• Motif Sites in GFF3--FIMO results showing the positions of occurrences of this discovered motif in the input sequences in GFF3 format. If the input sequences to MEME-ChIP have FASTA headers following the UCSC style ("chromosome_name:starting_position-ending_position"), and the chromosome names are in UCSC (not ENSEMBL) format, the GFF3 output will be suitable for uploading to the UCSC Genome Browser as a custom track.