DNA Damage Atlas



Basic Information

Dataset ID:

  PRJNA509652


PMID:

  31000663


Technique Type:

  ChIPseq + DISCOVER-seq


Publication:

  Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq


First Author:

  Beeke Wienert


Last Author:

  Jacob E Corn


Year:

  2019


Journal:

  Science


Access Link:   

Samples ( 23 )

  Sample Name    Cell Line/Tissue    Organism    Treatment    Genotype    In-situ Treatment    Cell Phase    Bait/Targeted Break    Technique    fastqc  
SRX5352597-BW15_hg38_k562_bfp_mre11K562Homo sapiens/////DISCOVER-seq
SRX5352612-BW16_hg38_k562_vegfa_mre11K562Homo sapiens////VEGFA site 2；gRNA sequences: GACCCCCTCCACCCCGCCTCDISCOVER-seq
SRX5352611-BW17_hg38_k562_emx1_mre11K562Homo sapiens////EMX1;gRNA sequences:GAGTCCGAGCAGAAGAAGAADISCOVER-seq
SRX5352608-BW19_hg38_k562_rnf2_mre11K562Homo sapiens////RNF2;gRNA sequences:GTCATCTTAGTCATTACCTGDISCOVER-seq
SRX5352589-BW42_mre11_k562_vegfa_xl_16hK562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCDISCOVER-seq
SRX5352617-BW43_mre11_k562_vegfa_5xl_16hK562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCDISCOVER-seq
SRX5352616-BW44_mre11_k562_bfp_36hK562Homo sapiens//// Non-targeting (BFP) ；gRNA sequences:GCTGAAGCACTGCACGCCATDISCOVER-seq
SRX5352604-BW49_mre11_ipscs_bfpiPSCsHomo sapiens//// Non-targeting (BFP) ；gRNA sequences:GCTGAAGCACTGCACGCCATDISCOVER-seq
SRX5352605-BW50_mre11_ipscs_nk1_mutiPSCsHomo sapiens////HSPB1-Mut；gRNA sequences: CGACTCGAAGGTGACTAGGADISCOVER-seq
SRX5352603-BW51_mre11_ipscs_nk2_wtiPSCsHomo sapiens////HSPB1-wt;gRNA sequences:CGACTCGAAGGTGACTGGGADISCOVER-seq
SRX5355580-Pilot_MRE11_BFPB16-F10Mus musculus//// Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATDISCOVER-seq
SRX5355578-Pilot_MRE11_GMB16-F10Mus musculus////Pcsk9-gM gRNA sequences:GGCTGATGAGGCCGCACATGDISCOVER-seq
SRX5355579-Pilot_MRE11_GPB16-F10Mus musculus////Pcsk9-gP gRNA sequences:AGCAGCAGCGGCGGCAACAGDISCOVER-seq
SRX5352598-BW15_hg38_k562_bfp_nbs1K562Homo sapiens////Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATChIP-seq (anti-NBS1)
SRX5352613-BW16_hg38_k562_vegfa_nbs1K562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCChIP-seq (anti-NBS1)
SRX5352593-BW15_hg38_k562_bfp_rad50K562Homo sapiens////Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATChIP-seq (anti-Rad50)
SRX5352610-BW16_hg38_k562_vegfa_rad50K562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCChIP-seq (anti-Rad50)
SRX5352596-BW15_hg38_k562_bfp_ku70K562Homo sapiens////Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATChIP-seq (anti-Ku)
SRX5352615-BW16_hg38_k562_vegfa_ku70K562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCChIP-seq (anti-Ku)
SRX5352595-BW15_hg38_k562_bfp_gh2axK562Homo sapiens////Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATChIP-seq (anti-γH2AX)
SRX5352614-BW16_hg38_k562_vegfa_gh2axK562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCChIP-seq (anti-γH2AX)
SRX5352602-BW15_hg38_k562_bfp_fd2K562Homo sapiens////Non-targeting (BFP)；gRNA sequences: GCTGAAGCACTGCACGCCATChIP-seq (anti-FANCD2)
SRX5352594-BW16_hg38_k562_vegfa_fd2K562Homo sapiens////VEGFA_site2;gRNA sequences:GACCCCCTCCACCCCGCCTCChIP-seq (anti-FANCD2)


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